Thin-Layer Chromatography

Thin-Layer Chromatography (TLC) is a simple, flexible and cost efficient separation technique for both qualitative and quantitative analysis, enabling simultaneous analysis of many substances with minimal time requirement. TLC can be performed manually in easy and inexpensive ways. Therefore it is found in almost all laboratories as a convenient tool for simple and rapid separations. As the expectations grow concerning quality and value of an analysis, there are suitable instruments available for all steps of TLC.

High-Performance Thin-Layer Chromatography (HPTLC) is the most advanced form of TLC and comprises the use of chromatographic layers of utmost separation efficiency and the employment of state-of-the-art instrumentation for all steps in the procedure: precise sample application, standardized reproducible chromatogram development and software controlled evaluation. HPTLC is an entire concept that includes a widely standardized methodology based on scientific facts as well as the use of validated methods for qualitative and quantitative analysis. HPTLC meets all quality requirements of today’s analytical labs, even in a fully regulated environment.

As opposed to other separation techniques, the inital costs for an HPTLC system as well as maintenance, and costs per sample are comparatively low. The possibility of visual evaluation of separated samples on the plate is one of the most valuable aspects of TLC. It reaches a completely new dimension in HPTLC through the use of state-of-the-art techniques for generating and evaluating digital images.

Description

Automated Multiple Development (AMD 2)

The CAMAG Automated Multiple Development procedure allows thin-layer chromatography to be utilized for tasks that could not be performed by TLC in the past.

Automatic Developing Chamber 2 (ADC 2)

The CAMAG Automatic Developing Chamber 2 (ADC 2) offers convenience, safety and reproducibility for isocratic developments of TLC/ HPTLC plates and foils with the format 20 x 10 cm.

Automatic TLC Sampler 4 (ATS 4)

Automatic sample application is a key factor for productivity of the HPTLC laboratory. The ATS 4 offers fully automatic sample application for qualitative and quantitative analyses as well as for preparative separations. It is suited for routine use and high sample throughput in mass an…

BioLuminizer®

The CAMAG BioLuminizer® is a detection system specifically designed to detect bioluminescence on HPTLC plates.

Chromatogram Immersion Device

For proper execution of the dipping technique, the chromatogram must be immersed and withdrawn at a controlled uniform speed. By maintaining a well defined vertical speed and immersion time, derivatization conditions can be standardized and «tide marks», which can interfere with densito…

Derivatizer

Spraying device for the automated derivatization of TLC/HPTLC plates

Flat Bottom Chamber

The CAMAG Flat Bottom chamber permits the chromatogram to be developed under conditions of partial or complete saturation of the tank atmosphere with solvent vapors. The degree of layer pre-saturation can not be controlled unless additional accessories are used.

Horizontal Developing Chamber

In the CAMAG Horizontal Developing Chamber, a plate can be developed in the sandwich as well as in the tank configuration. The chamber is suitable for all kinds of solvents.

HPTLC Vario System

Development with six different solvents can be tested side by side

Linomat 5

With the CAMAG Linomat 5 samples are sprayed onto TLC/HPTLC plates in the form of bands with nitrogen or compressed air. Sample application is automatic, only changing the syringe (filling, inserting and rinsing) is manual. The Linomat is suitable for routine use.

Nanomat 4 and Capillary Dispenser

The CAMAG Nanomat 4 serves for easy application of samples in the form of spots onto TLC and HPTLC layers, precisely positioned and without damage to the layer.

Reagent Sprayer

This all glass reagent sprayer is a low cost alternative to the TLC/HPTLC Sprayer. It comes with a rubber pump but may also be operated from a compressed air or nitrogen supply.

smartALERT Solvent Front Monitor

CAMAG smartALERT for dependable monitoring of TLC/HPTLC plate development in a glass development chamber.

smartCUT Plate Cutter

Convenient and precise cutting of pre-coated TLC/HPTLC glass plates.

Test Dye Mixtures

Test dye mixtures are useful for functional checks on individual steps in the TLC procedure and for studying the influence of specific parameters.

TLC Plate Heater

The CAMAG TLC Plate Heater is designed for heating TLC plates to a selected temperature after a staining reagent has been applied.

TLC Scanner 4

The CAMAG TLC Scanner 4 is the most advanced workstation for densitometric evaluation of TLC/HPTLC chromatograms and other planar objects.

TLC Spray Cabinet

The CAMAG TLC Spray Cabinet ensures the complete removal of reagent mist while spraying TLC plates.

TLC Visualizer 2

Professional imaging and documentation system for TLC/HPTLC chromatograms with a new digital CCD camera, connected by USB 3.0

TLC/HPTLC Pre-coated plates

In most cases modern TLC utilizes precoated layers. Not only are they more convenient, their quality is superior to that of layers prepared from the type of adsorbents available for self-coating.

TLC/HPTLC Sprayer

The TLC/HPTLC Sprayer consists of a charger and a pump unit with two kinds of spray heads. Spray head type A is for spray solutions of normal viscosity, e.g. lower alcohol solutions. Spray head type B is for liquids of higher viscosity, e.g. sulfuric acid reagents.